Journal of Biotechnology and Biomedical Science

Journal of Biotechnology and Biomedical Science

Current Issue Volume No: 2 Issue No: 1

Research-article Article Open Access
  • Available online freely Peer Reviewed
  • Phytochemical Analysis And Thin Layer Chromatography Profiling Of Crude Extracts From Senna Occidentalis(Leaves)

    A.M Lawal 1
        R Abdullahi 1     M.S Ibrahim 2     M.Y Kurfi 3     A Khalid 4     M Nuhu 1    

    1 Department of Chemistry, Federal University Dutse, Jigawa State, P.M.B 7156 

    2 Department of Polymer and Textile Engineering, Ahmadu Bello University, Zaria, Nigeria 

    3 Department of Pure and Industrial Chemistry, Bayero University Kano 

    4 Science Department, Great Icon International School, Zaria 

    Abstract

    Plants used for medicinal practices which were discovered since prehistoric stone ages are termed Medicinal plants, which are also referred to as medicinal herbs, since plants produces bioactive chemical compounds (phytochemicals), this research however, is concerned with the extraction using Soxhlet extraction technique, phytochemical screening using various test methods, which reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for extracts of senna occidentalis and also, thin layer chromatography profiling which gives probable foundation for further structural elucidation amongst others. This research shows the presence of potent secondary metabolites present in the leaves of senna occidentalis (leaves).

    Author Contributions
    Received Apr 22, 2019     Accepted May 13, 2019     Published May 29, 2019

    Copyright© 2019 A.M Lawal, et al.
    License
    Creative Commons License   This work is licensed under a Creative Commons Attribution 4.0 International License. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.

    Funding Interests:

    Citation:

    A.M Lawal, R Abdullahi, M.S Ibrahim, M.Y Kurfi, A Khalid et al. (2019) Phytochemical Analysis And Thin Layer Chromatography Profiling Of Crude Extracts From Senna Occidentalis(Leaves) Journal of Biotechnology and Biomedical Science. - 2(1):12-21
    DOI 10.14302/issn.2576-6694.jbbs-19-2791

    Introduction

    Introduction

    A medicinal plant is any plant material be it seeds, root extracts or leaves extract that is used to cure or fight against infection or attempt to maintain health, which are to be administered for specific ailments which can either be in modern or traditional medicine, this definition of medicinal plant is also supported by Ahn, 12. There are about 50,000 medicinal plants used across the world 3.It is documented by Kew (2016) that there are conservatively about 18,000 plant species that are use either in modern or traditional ways which are conceived to have medicinal properties which are part of the about 30,000 species documented.

    Medicinal plants provide various kinds of benefit which could either be economic, health or socio-cultural benefits as suggested by Schippmann et al., 3. However, development of plants or extracts and available expertise to prove that they have potential medicinal uses is blunted and weakened, upon which insufficient financing is a key player,1.

    Drug development researchers adopts ethno botany as a strategy to search for pharmacologically active substances in naturally occurring plants, using these strategies, they have discovered hundreds and thousands of useful compounds which can either be alkaloids, glycosides, polyphenols, or terpenes. Some of the drugs extracted from plants include aspirin, quinine, opium, etc.

    Bioactive Chemical Compounds (Phytochemicals)

    All plants produce chemical compounds which give them an evolutionary advantage, such as defense against herbivores or, in the example of salicylic acid, as a hormone in plant defenses, 45. These phytochemicals have potential uses as drugs, and the content of known pharmacological activity of these substances in medicinal plants is the scientific basis for their use in modern medicine, if scientifically confirmed, 1.

    Modern knowledge of medicinal plants is being systematized in the Medicinal Plant Transcriptomics Database, which by 2011 provided a sequence reference for the transcriptome of some thirty species, 6. The major classes of pharmacologically active phytochemicals are described below, with examples of medicinal plants that contain them, 7.

    Alkaloids

    Alkaloids are bitter-tasting chemicals, very widespread in nature, and often toxic, found in many medicinal plants, 8. There are several classes with different modes of action as drugs, both recreational and pharmaceutical. Medicines of different classes include atropine, scopolamine, and hyoscyamine (all from nightshade), 910.

    Glycosides

    Anthraquinone glycosides are found in medicinal plants such as rhubarb, cascara, Aloe and Alexandrian senna, 9111213. The cardiac glycosides are powerful drugs from medicinal plants including foxglove and lily of the valley. They include digoxin and digitoxin which support the beating of the heart, and act as diuretics, 5.

    Polyphenols

    Polyphenols of several classes are widespread in plants, having diverse roles in defenses against plant diseases and predators, 5. They include hormone-mimicking phytoestrogens and astringent tannins, 914. Plants containing phytoestrogens have been administered for centuries for gynecological disorders, such as fertility, menstrual, and menopausal problems, 1516171819.

    Terpenes

    Terpenes and terpenoids of many kinds are found in a variety of medicinal plants, 18 and in resinous plants such as the conifers. They are strongly aromatic and serve to repel herbivores. Their scent makes them useful in essential oils, whether for perfumes such as rose and lavender, or for aromatherapy, 92021. Some have medicinal uses: for example, thymol is an antiseptic and was once used as a vermifuge (anti-worm medicine), 21.

    Cassia Occidentalis

    S. occidentalis is an annual too short-lived perennial herb to small shrub with a pantropical distribution. It is reported as invasive throughout Oceania, and various countries in Asia and Africa. Within its native range S. occiden-alis is listed as invasive for Cuba by Oviedo Prieto et al. 22. The scientific name is Senna occidentalis with a common name as Coffee Senna.

    Taxonomy

    Domain: Eukaryota

    Kingdom: Plantae

    Phylum: Spermatophyta

    Subphylum: Angiospermae

    Class: Dicotyledonae

    Order: Fabales

    Family: Fabaceae

    Subfamily: Caesalpinioideae

    Genus: Senna

    Species: Senna Occidentalis

    Description

    The morphology of S. occidentalis as described by Parsons and Cuthbertson 23, closely matches that as described in other places in the world. It is a low growing, sparsely branching annual or short-lived perennial plant up to 0.5-2 m high and having a characteristic fetid odour. The stems are reddish purple, erect, 4-angled when young, becoming rounded with age. The plant has a robust primary root with several laterals. The leaves are pale green on reddish stalks; alternate, pinnate, with 3-5 (sometimes 6) pairs of opposite ovate to lanceolate-elliptic leaflets, 25-100 mm long, 20-30 mm wide, rounded at the base. A conspicuous, dark-coloured gland occurs at the base of the petiole (leaf stalk) but not on the stalks of the leaflets. The flowers are pale to bright yellow, 20-30 mm in diameter, in 2-6 flowered axils of the upper leaves; sepals are red veined; 5 petals per flower, the 2 anterior ones are smaller than the others; fertile stamens 6, the two basal ones longer than the rest, 4 infertile stamens are reduced to tiny petal-like staminodes. Further descriptions of the floral anatomy of S. occidentalis and nine other species of Cassia are given by Chhavi-Thakur and Thakur 24. The fruit is a dark brown, flattened, sickle-shaped pod with paler stripes along the edges when mature. Pods are 75-130 mm long, 8-10 mm wide, containing a single row of 25-35 seeds. The seeds are dark brown, flattened, hard, 5 mm long and 3 mm wide. Plate 1

    Close view of senna occidentalis in its natural habitat
    Phytochemistry

    Phytochemical screening of the plant showed the presence of carbohydrates, saponins, sterols, flavonoids, resins, alkaloids, terpenes, anthraquinones, glycosides and balsam. Presence of these metabolites strongly concluded the great potential of the plant as a source of phytomedicines. As the flavonoids and resins are present, it might be responsible for its anti-inflammatory properties. Chinese folkloric medicine contains flavonoids which has anti-inflammatory effect on both acute and chronic inflammation, alkaloids for blood pressure decrease and nervous system balancing, Tannins for wound healing and terpenes with anti-viral properties, 252627.

    Eudesmane sesquiterpenes have been reported to contain antibacterial properties. Saponins are believed to have antioxidant, anti-cancer, anti-inflammatory, and anti-viral properties. The anthraquinones, emodin and chrysophanone have been reported to possess wound healing properties. Other compounds reported in literature include, 1,8-dihydroxyl-2-methyl anthraquinone, 1,4,5-trihydroxy-3-methyl-7-methoxy anthraquinone, cassiaoccidentalin A, B and C, which are C-glycosides, achrosine, anthrones, apigenin, aurantiobtusin, campesterol, cassiollin, chryso-obtusin, chrysophanic acid, chrysarobin, chrysoeriol, essential oils, funiculosin, galactopyranosyl, helminthosporin, islandicin, kaempferol, lignoceric acid, linoleic acid, linolenic acid, mannitol, mannopyranosyl, matteucinol, obtusifolin, obtusin, oleic acid, physcion, quercetin, rhamnosides, rhein, rubrofusarin, sitosterols, and xanthorin 2829. Figure 1

    Structures of some compounds isolated from Senna occidentalis

    Results

    Results and Discussion

    Results of extraction, phytochemical screening, free radical scavenging and TLC profiling of the leaves of Senna Occidentalis. Table 2

    Result of Extraction
    SN SAMPLE WEIGHT USED (g) SOLVENTS USED WEIGHT OF CRUDE EXTRACT(g)
    1 Senna Occidentalis/Leaves 400 Hexane 33.20
          Diethyl Ether 3.00
          Chloroform 12.40
          Ethyl Acetate 3.80
          Methanol 24.80

    Discussion

    Discussion

    From the above results, the weight of the crude extracts was found to be 33.20, 3.00, 12.40, 3.80, 24.80, (g) for hexane, diethyl ether, chloroform, ethyl acetate and methanol extract of sennaoccidentalis leaves with hexane having the highest mass using the aforementioned successive soxhlet extraction technique.

    The preliminary phytochemical screening reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponnins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for various extracts of sennaoccidentalis using various tests. (Table 3). This may account for their various uses in ethno-botanical and traditional medicines.

    Preliminary phytochemical analysis of leaves extract obtained from S. occidentalis
    Metabolites Test Used Leaves Extract
        N-HX DE CF EA ME
    Anthraquinones Free anthraquinones Combined anthraquinones General test   - - - + +
    - - - +
    Alkaloids Dragendoff s test - - - - -
    Mayer s test - - - - -
    Wagner s test - - - - -
    Carbohydrates Molisch s test + - - - +
    Cardiac glycosides Kella-Killani test + + + - -
    Glycosides FeCl3 test - - - - +
    Flavonoids Shinoda test - - - - +
    NaOH test - - -   +
    Sulphuric acid test - - - - +
    Lead acetate test - - - - +
    Saponnins Frothing test - - - + +
    Steroids/ Terpenes Liebermann-Buchard test + + + + +
    Salkowski test + + + + +
    Phenolic compounds and tannins FeCl3 test - - - - +

    Also, the TLC profiling was carried to know the solvent system that could possibly be used in the further isolation of the crude extract from the plants. Although, an ascending chromatography it gives an idea about the possible compounds that can be present in the extract from the number of spots (Table 4), and also, the possible solvent mixture that could be used for further isolation.

    Result of Thin Layer Chromatography (TLC) Profiling using solvent system comprising hexane/ethyl acetate mixtures (99, 98, 97, 96, 95, 93, 91, 90, %)
    SN SOLVENT PLANT SPOT COLOUR 10% H2SO4
    1 Hexane Senna Occidentalis 1,2 & 3 Purple, Yellow & Dark green
    2 Diethyl ether Senna Occidentalis 1 & 2 Orange & Yellow
    3 Chloroform Senna Occidentalis 1,2,3 & 4 Green, Light blue, Yellow & Black
    4 Ethyl acetate Senna Occidentalis 1,2 & 3 Black, Yellow & Dark green
    5 Methanol Senna Occidentalis 1,2,3 & 4 Purple, Yellow, Dark red & Blue

    Conclusion

    Conclusion

    From the above premises it can be concluded that, after the extraction of the crude extracts, the leaves of sennaoccidentalis were found to be rich in phytochemicals. Also from relevant literatures, it clearly indicates that, the leaves extracts of Senna occidentalis houses some pure compounds whose structures can be further elucidated and be used for drug design. This means that the leaves would be useful as an antioxidant and free radical scavenging agent and also aids in the treatment of many diseases mediated by reactive oxygen species amongst others.

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