Abstract
Plants used for medicinal practices which were discovered since prehistoric stone ages are termed Medicinal plants, which are also referred to as medicinal herbs, since plants produces bioactive chemical compounds (phytochemicals), this research however, is concerned with the extraction using Soxhlet extraction technique, phytochemical screening using various test methods, which reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for extracts of senna occidentalis and also, thin layer chromatography profiling which gives probable foundation for further structural elucidation amongst others. This research shows the presence of potent secondary metabolites present in the leaves of senna occidentalis (leaves).
Author Contributions
Copyright© 2019
A.M Lawal, et al.
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing interests The authors have declared that no competing interests exist.
Funding Interests:
Citation:
Introduction
A medicinal plant is any plant material be it seeds, root extracts or leaves extract that is used to cure or fight against infection or attempt to maintain health, which are to be administered for specific ailments which can either be in modern or traditional medicine, this definition of medicinal plant is also supported by Ahn, Medicinal plants provide various kinds of benefit which could either be economic, health or socio-cultural benefits as suggested by Schippmann Drug development researchers adopts ethno botany as a strategy to search for pharmacologically active substances in naturally occurring plants, using these strategies, they have discovered hundreds and thousands of useful compounds which can either be alkaloids, glycosides, polyphenols, or terpenes. Some of the drugs extracted from plants include aspirin, quinine, opium, etc. All plants produce chemical compounds which give them an evolutionary advantage, such as defense against herbivores or, in the example of salicylic acid, as a hormone in plant defenses, Modern knowledge of medicinal plants is being systematized in the Medicinal Plant Transcriptomics Database, which by 2011 provided a sequence reference for the transcriptome of some thirty species, Alkaloids are bitter-tasting chemicals, very widespread in nature, and often toxic, found in many medicinal plants, Anthraquinone glycosides are found in medicinal plants such as rhubarb, cascara, Aloe and Alexandrian senna, Polyphenols of several classes are widespread in plants, having diverse roles in defenses against plant diseases and predators, Terpenes and terpenoids of many kinds are found in a variety of medicinal plants, Domain: Eukaryota Kingdom: Plantae Phylum: Spermatophyta Subphylum: Angiospermae Class: Dicotyledonae Order: Fabales Family: Fabaceae Subfamily: Caesalpinioideae Genus: Species: The morphology of Phytochemical screening of the plant showed the presence of carbohydrates, saponins, sterols, flavonoids, resins, alkaloids, terpenes, anthraquinones, glycosides and balsam. Presence of these metabolites strongly concluded the great potential of the plant as a source of phytomedicines. As the flavonoids and resins are present, it might be responsible for its anti-inflammatory properties. Chinese folkloric medicine contains flavonoids which has anti-inflammatory effect on both acute and chronic inflammation, alkaloids for blood pressure decrease and nervous system balancing, Tannins for wound healing and terpenes with anti-viral properties, Eudesmane sesquiterpenes have been reported to contain antibacterial properties. Saponins are believed to have antioxidant, anti-cancer, anti-inflammatory, and anti-viral properties. The anthraquinones, emodin and chrysophanone have been reported to possess wound healing properties. Other compounds reported in literature include, 1,8-dihydroxyl-2-methyl anthraquinone, 1,4,5-trihydroxy-3-methyl-7-methoxy anthraquinone, cassiaoccidentalin A, B and C, which are C-glycosides, achrosine, anthrones, apigenin, aurantiobtusin, campesterol, cassiollin, chryso-obtusin, chrysophanic acid, chrysarobin, chrysoeriol, essential oils, funiculosin, galactopyranosyl, helminthosporin, islandicin, kaempferol, lignoceric acid, linoleic acid, linolenic acid, mannitol, mannopyranosyl, matteucinol, obtusifolin, obtusin, oleic acid, physcion, quercetin, rhamnosides, rhein, rubrofusarin, sitosterols, and xanthorin
Results
Results of extraction, phytochemical screening, free radical scavenging and TLC profiling of the leaves of Senna Occidentalis.
SN
SAMPLE
WEIGHT USED (g)
SOLVENTS USED
WEIGHT OF CRUDE EXTRACT(g)
1
400
Hexane
33.20
Diethyl Ether
3.00
Chloroform
12.40
Ethyl Acetate
3.80
Methanol
24.80
Discussion
From the above results, the weight of the crude extracts was found to be 33.20, 3.00, 12.40, 3.80, 24.80, (g) for hexane, diethyl ether, chloroform, ethyl acetate and methanol extract of The preliminary phytochemical screening reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponnins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for various extracts of n-HX= n-Hexane, DE= Diethyl Ether, CF= Chloroform, EA= Ethyl Acetate, ME= Methanol Also, the TLC profiling was carried to know the solvent system that could possibly be used in the further isolation of the crude extract from the plants. Although, an ascending chromatography it gives an idea about the possible compounds that can be present in the extract from the number of spots (
N-HX
DE
CF
EA
ME
Anthraquinones Free anthraquinones Combined anthraquinones
General test
+
+
-
-
-
+
+
Alkaloids
Dragendoff s test
Mayer s test
Wagner s test
-
-
-
-
-
Carbohydrates
Molisch s test
Cardiac glycosides
Kella-Killani test
Glycosides
FeCl3 test
+
Flavonoids
Shinoda test
NaOH test
-
+
Sulphuric acid test
-
-
-
-
+
Lead acetate test
-
-
-
-
+
Saponnins
Frothing test
Steroids/ Terpenes
Liebermann-Buchard test
Salkowski test
+
+
+
+
+
Phenolic compounds and tannins
FeCl3 test
SN
SOLVENT
PLANT
SPOT
COLOUR 10% H2SO4
1
Hexane
Senna Occidentalis
1,2 & 3
Purple, Yellow & Dark green
2
Diethyl ether
Senna Occidentalis
1 & 2
Orange & Yellow
3
Chloroform
Senna Occidentalis
1,2,3 & 4
Green, Light blue, Yellow & Black
4
Ethyl acetate
Senna Occidentalis
1,2 & 3
Black, Yellow & Dark green
5
Methanol
Senna Occidentalis
1,2,3 & 4
Purple, Yellow, Dark red & Blue
Conclusion
From the above premises it can be concluded that, after the extraction of the crude extracts, the leaves of