Abstract
In Niger, sorghum (Sorghum bicolor) is one of the most important staple crop for food and money. Despite the importance of the crop and its easiest adaptative capacity to dry, the crop is confronted to striga (Striga hermonthica), a plant parasite, which drastically hinder the productivity and causing yield loss between 20% to 80%. The best and easiest way to control and improve sorghum productivity is through the development of novel Striga resistance sorghum genotypes. The purpose of this study was to assess 20 sorghum genotypes from diverse country for striga resistance and grain yield capacity. Varieties with high yield average, medium size and resistance to Striga were found.
Author Contributions
Copyright© 2023
Abdou Ousseini Ardaly, et al.
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing interests The authors have declared that no competing interests exist.
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Introduction
In Niger, sorghum is mainly grown in harsh and dry environmental conditions, where rainfall is less than 200 to 300 mm per year Nonetheless, to inhibit Striga impact on sorghum, several resistance mechanisms have been successfully identified Thus, with the increase of Striga impact around the world, and Striga races variability, there are some sorghum genotypes which can better perform under high Striga infestation and give good yield better than other genotypes. In addition, under the same conditions, some genotypes cannot perform well. Some sorghum genotypes are highly susceptible to the weed resulting in grain yield reduction or a complete yield loss The objectives of this study were to evaluate the twenty sorghum genotypes for Striga resistance, to identify a medium sorghum genotypes and grain yield capability estimation under Striga field infestation.
Materials And Methods
The field trial was conducted in Konni region at Wazir, INRAN Striga screening field located around 8 km from Konni and 3 km from the main road (RN1). Konni is located around 417 km from Niamey in the eastern part of Niger with a rainfall distribution of between 500 to 600 mm (INS, 2016). Wazir is an area characterized by an agricultural farming system where farmers grow different cash crops. It is a highly Striga infested area and was used since 1987 for Striga screening purposes by INRAN Niger. Twenty sorghum genotypes ( The data on sorghum crop components and Striga traits were collected in field. The sorghum data included plant vigor (Vg), 50% days to flowering (50% Flo), number of sorghum plants per hill (NPSorg), number of panicles (NPANI), plant height (HTR) in cm, 1000 grain weight (PoiGR) in g, and grain yield (Kg). The Striga emergence (EMR), the number of Striga plants emerged at 45, 60 and 90 days (SN45, SN60 and SN90) were collected as Striga related traits. The recorded data were transferred and analyzed using SAS (9.4). Thus, a normality test was performed in GENSTAT Edition 14, following by general linear model analysis and principal component analysis using SAS (9.4). Highly significant: **; Significant * EMR=Striga emergency; Flo= 50% flowering date; HTR = Plant height; NPANI= Panicles number; NPSorg=Hill number; NS45= Number of Striga plants at 45 days after planting date; NS60= Number of Striga plants at 60 days after planting date; NS90= Number of Striga plants at 90 days after planting date; PoiGR= 1000 grain weight; Vig= Plant vigor; Yield = grain yield Kg/ha; ns=non- significant
SRN39
Sudan
2
Striga resistant (Low strigolactone production)
El mota
Niger framers preferred land race
1.5
Early maturing/ unknown tolerance to Striga
P9401
SRN39X P954063
4
Striga resistant and early maturing
P9403
SRN39X P954063
3
Striga resistant and early maturing
Brahan
(Framida×SRN39) United States
/
Striga resistant
S35
ICRISAT
/
Striga resistant
F2-20
(MN1056 x 68-20) x 7410-195-1
3.5-5.0
Striga resistant
CE -151- 262
CE 90 x 73-71 (IS 12610)
2.0 - 4.3
Striga resistant
04-CZ-F5 P-52
Burkina
/
Striga resistant
ICSV- 1049
Burkina
5
Striga resistant
MDK (Matche Da Koumya)
Niger framers preferred land race
3
Good yield, late mature variety
CE -180-33
7455 (Senegal lines ) x Naga white (Ghana)
2.8 - 5
Striga resistant
P9406
SRN39X P954063
/
Striga resistant and early maturing
P9405
SRN39X P954063
3.0 - 4.0
Striga resistant and early maturing
Mota Maradi
Niger framers preferred land race
2
Early maturing
Lignee 16
(TXN13)BC3F5-41
4.0 - 5.0
Striga resistant and good yield
Lignee 17
(AG-8XN13)BC3F5
5
Striga resistant and Good yield
Framida
ICRISAT
3.7
Striga resistant (Low strigolactone production)
Hakori Karoua
Niger framers preferred land race
1.0-1.5
Good yield
El tsedaoua
Niger framers preferred land race
1.5
Early maturing
-
0.48 ns
-
0.00 ns
0.13ns
-
0.12 ns
0.91**
0.02 ns
-
0.01 ns
0.003 ns
0.68**
0.09 ns
-
0.03 ns
0.36 ns
0.33 ns
0.45 ns
0.03 ns
-
-0.69**
0.30ns
0.004 ns
0.26 ns
0.003 ns
0.79**
-
-0.69**
0.30 ns
0.005 ns
0.14ns
0.004 ns
0.81**
0.98**
-
0.41ns
0.86**
0.08 ns
0.80**
0.09 ns
0.77**
0.58 ns
0.52 ns
-
0.03ns
0.04
0.34 ns
0.35 ns
0.38 ns
0.26 ns
0.22 ns
0.22 ns
0.47 ns
-
0.71**
0.84**
0.58ns
0.05 ns
0.18 ns
0.41 ns
0.621*
0.75**
0.77 **
0.88**
-
Results
Linear correlation among traits varied from none, significant and highly significant correlations values. Number of Striga plants emerged after planting was negatively and highly correlated with number of Striga plants emerged at 60 days (r = -0.69) and Striga plants emerged at 90 days (r=-0.69). Thus, for the 50% days to flowering (Flo), a high significant correlation was observed with the panicles number, the grain weight, and the grain yield with respectively a correlation value r = 0.91, r = 0.86 and r = 0.84 ( From the screening at Konni station, a principal component analysis (PCA) using 11 traits was performed to assess 20 sorghum genotypes from different countries. Among those 11 traits used, only the component 1, the component 2 and the component 3 were identified to have an eigen value higher (>) than 1 under Striga infestation condition at Konni station. Thus, the three components contribute at 42.3% for the component 1, 14.4% for the component 2 and 79.1% for the component 3, in the total phenotypic variation among the 20 genotypes. The selected three Eigen values are explaining around 79.1% of the variation among the collected parameters in the experiment. Therefore, depending on the correlation with the 11 traits (Sorghum and Striga traits) initially used, several significant correlation matrices were obtained ( Eigen value ˃1; * Significant correlation; The component plotting scores gave the results of the first two components, PC1 and PC2 on the genotypes distribution. In addition, each component was distributed into four environments comprising the environment 1 to the environment 4 ( Concerning the component one and the component three, at this side, the environment 1 of the component 1 contain the genotype SRN39, CE-180-33, El tsedaoua and were positively correlated to the environment one. Thus, in the environment 2 correlated positively there are six genotypes represented by the genotype F2-20, 04-CZ-F5P-52, ICSV-1049, MDK, FRAMIDA, Hakori Karoua. For instance, the environment 3 was positively and negatively correlated respectively to the component 1 and the component 3 with the genotypes P9401, P 9403, Brahan, S35, CE-151-262 and the genotype P9405. In addition, the last environment correlated negatively to the component 1 and the component 3, four genotypes, were found, the SRN39, P9406, Mota Maradi and (AG-8XN13) BC3F5. For the component two and the component three, the environment 1 was positively correlated to the component 2 and contain the genotype El Mota and El tsedaoua. Concerning the environment 2 of the component 2 was positively correlated with the genotype P9401, P9403, F2-20, CE-151-262, and the genotype (TXN13) BC3F5-41. Thus, the environment 3 is negatively correlated to the component 2 and positively correlated to the component 3 and contain Brahan, S35, 04-CZ-F5P-52, ICSV1049, MDK, P9405, FRAMIDA, Hakori Karoua .
Variables
PC1
PC2
PC3
Plant vigor
-0.17
0.08
0.61*
Striga emergence
-0.36*
0.07
0.11
Number of Striga plant at 45 days
0.32*
-0.28
-0.04
Number of Striga plant at 60 days
0.41*
-0.19
-0.05
Number of Striga plant at 90 days
0.41*
-0.172
-0.05
Number of sorghum plant hill
0.36*
0.05
0.24
50% flowering date
0.15
-0.09
0.71*
Plant height
0.36*
0.11
0.12
Panicle number
0.24
0.41*
-0.11
1000 grains weight
0.18
0.57*
-0.04
Grain yield
0.05
0.55*
0.04
Eigen value
4.75
2.36
1.58
%variance
42.3
21.4
64.7
Cumulative% variance
42.3
14.4
79.1
Discussion
The main objective of the study was to assess 20 sorghum genotypes under Striga infestation at Konni station in Niger for Striga resistance and determine Striga impact on sorghum growth and agronomic parameters. Thus, the normality test performed was significant along the recorded data. In addition, the general linear analysis (GLM) with the different mean squares obtained on the sorghum attributes and the weed parameters, were also significant. Moreover, this obtained result show a large variation among the genotype s and confirm the existence variability between them. The correlation study undertaken sort the interaction between the different variables involved in the experiment to better guide the breeders. For instance, correlation study deeply gave more information on the bidirectional relationship of the characters Eigen values greater than one. Thus, the varieties located in the environment 3 of the component 2 have a higher negative correlation with the component 1. However, in this environment Striga emergence starts earlier compared to in the other s environments 3. That means the genotype P9401, P9403, Brahan, S35, CE-151-262 and P9405 were under high Striga emergence impact with high grain yield. The highest plant height (HTR) were located in the environment 2 of the component 1. In this environment (ENV2), the genotype El Mota, F2-20 and El tsedaoua possess a high height compared to the others genotypes. The component 2 was positively correlated to the number of panicles (NPANI), the 1000 grain weight (PoiGR) and the grain yield in the environment 3 with high Striga emergence impact and concern the genotype P9401, (TXN13) BC3F5-41, CE-151-262 and P9403. In the environment 3 of the component 3 positively correlated to the plant vigor and the 50% date to flowering (Flo), the Striga emergence was high in this area and contains the genotype P9401, P9403, Brahan, S35, CE151-262, 04-CZ-F5P-52, ICSV-1049, MDK, P9405, (TXN13) BC3F5-41, FRAMIDA, and Hakori Karoua. Those varieties are characterized by a better plant vigor and are early flowering genotypes. In addition, the component 3, was also negatively correlated to the number of Striga plants number at 45, 60 and 90 days after the planting date. It concerned SRN39, CE-180-33, P9406, Mota Maradi and the genotype (AG-8XN13) BC3F5 highly infested by the weed with less grain yield observed. This environment contains a high Striga plants number and confirmed the presence of tolerant genotypes with high grain yield in these area.
Conclusion
The present study made on the assessment of striga impact on sorghum attributes and striga traits, put in light the variability among the twenty sorghum genotypes and their performance’s against the weed. The PCA analysis used, have grouped the different variables according to their correlations and their respective comportments. So through the obtained results on the three components, we found that the genotype P9401, P9403, Brahan, S35, CE 151-262, P406, and TXN13/BC3F5-41 are high yielding genotypes with medium height, good panicles numbers, good grain weight are resistant to the weed. Those promising genotypes can be easily used in a hydration breeding program for improving some local land races highly susceptible to striga but highly appreciated by smallholder’s farmers.