Experiments were conducted in the laboratory of the College of Agricultural Studies - Sudan University of Science&Technology, Shambat, Khartoum- Sudan during 2018-2019,where the extracts of four plants, Neen (Azadirachta indica), Black pepper (Piper nigrum ), Usher (Calotropis procera ) and Argel (Solenostemma argel), were chosen for application against immature stages of FAW (S. frugiperda). Neem seeds and Usher leaves were collected from Shambat area, and Argel leaves and Black pepper were obtained from the local market. All these materials were washed and dried under laboratory conditions for 48 hrs. Then, they were ground to a fine powder by an electric blender (Moulinex), and the powders were kept in tight containers to be used later.

Neem seeds, Black pepper seeds and Usher leave powder extracts were prepared by using Ethanol according to the method described by Sukhdev et al. (2008)11. Samples of each plant powder were soaked with absolute Ethanol. Extraction was carried out for three days, with daily filtration and evaporation of the solvent under reduced pressure using rotary evaporator apparatus. Samples were exposed to air in an evaporating dish till complete dryness. A stock concentration was prepared for each extract and serial dilutions were made to prepare 4 different concentrations for the bioassay treatments.

Moreover, aqueous extraction was also carried out according to the method of 11, with slight modification. The extract was prepared by mixing 2.5gm of the leaves powder in 10 liters of water, and the mixture was left for 24 hrs. Then, it was strained through a cotton cloth and kept as a stock solution for later use. Also, 4 concentrations were prepared for the bioassay tests.

For preparing laboratory bioassays, four groups, each of 15 recently hatched larvae of (FAW) were placed in Petri-dishes, each contained a piece of fresh maize leaf which previously immersed for 5 seconds in each of the four different concentrations (10, 25, 50 and 75%) of each extract. Another group, of 15 larvae, was used as a control with each concentration, and was placed in a Petri-dish contained fresh maize leaf, treated with Ethanol, and with distilled water with Argel extract. A group of 15 larvae was added to each replicate, and was treated with the recommended dose of a standard pesticide, Spinosad (Tracer Spinosad, Chemimport Company Ltd, Sudan). The experiments were arranged in a complete randomized block design experiment (CRBD), and the test containers were kept in the laboratory, at a temperature of (27±2) ⁰c and a R.H. of (56±2)%. Each treatment was replicated three times. Larval mortality was recorded after 24, 48 and 72 hrs. Criteria of larval mortality: the larval color changed from brownish to dark, then larva became sluggish, and finally died. The collected data were analyzed by Analysis of Variance (One Way ANOVA) using SPSS program (version 20), and the means were separated using Tukey test.

In this study the bioassay tests showed the ethanolic extracts of the three plant species, the Neem, Black Pepper and Usher, were effective against the FAW larvae.

The extensive studies carried out during the last decades proved the potential of plant extracts as alternative insect pest control agents 1213. Concerning the FAW, Rioba, et al.; (2019) in their review stated that, 69 plant species were found as effective control agents against FAW in various parts of the world. Likewise, In the present study the results of the bioassay tests showed that, the ethanolic extracts of the three plant species, e.g. Neem, Black Pepper and Usher, (shown in Table1, table 2, table 3) were effective against the FAW larvae.

Considering table 1, the highest concentration of Neem ethanolic extract (75%) caused 100% larval mortality after 72 hrs of exposure, which was significantly different compared with other concentrations. However, it was not significantly different from the recommended dose of Spinosad. This result is almost in full agreement with those of Sisay et al.9, who stated that, three botanical extracts, including Neem showed equal efficacy with that of four synthetic insecticides against larvae of FAW after72 hours.

The above table 2, the efficacy of the Black pepper extracts showed that, the two high concentrations (75 and 50%) also caused 100% larval mortaliy of FAW, which was comparable to that of the standard pesticide spinosad. These results are also comparable to that of Celis et al. 12 who mentioned that, methanolic extracts of six Piper species caused larval mortality of FAW similar to that obtained by the insecticide Chlorpyrephos.

The results of bioassay tests of Usher leaves extract shown in table 3 indicated that almost all concentrations caused 100% larval mortality of FAW similar to that of Spinosad. This efficacy of Usher extracts is similar to that of Santos 13. Moreover, Rioba et al. 14 showed that, morality of FAW larvae increased by feeding on maize leaves impregnated by Usher leaves extracts.

On the other hand, the results of table 4 indicated that the Argel water extract was not effective on FAW larvae.

The present study is the first one that indicated the effectiveness of three plants’ extracts against FAW in Sudan. The results obtained by their highest concentrations after 72 hours were comparable to that of the standard insecticide Spinosad. The strong insecticidal activity of Spinosad against many insect pests, particularly of Lepidoptera, was reported in previous studies by Salgado15 and Huang et. al 16.

The extensive studies carried out during the last decades proved the potential of plant extracts as alternative insect pest control agents 1718. Concerning the FAW, Rioba et al. 14 in their review stated that, 69 plant species were found as effective control agents against FAW in various parts of the world.

Based on the results of the present study,which showed the equal efficacy of the plant extracts and the pesticide Spinosad, it is worth considering the costs of the FAW control by each group, and the impact on the Environment. The price of “100 ml of Spinosad” in the Pesticide Market in Khartoum State equals to 750 SDG, while the price of one Pound of Black pepper in the local market is equal to 500 SDG. In comparison, Tens of Kgs of Neem seeds, or of Usher leaves, can be collected “free of charge “at any time from the open fields in Khartoum State.