Abstract
Genotype by environment (GE) interaction is considered to be among the major factors limiting the efficiency of breeding programs.Five chickpea genotypes were evaluated to study their adaptability and stability in eight environments of Sudan. The experiments were carried out in randomized complete block design (RCBD) with four replications in four locations in over two years (2017/2018 and 2018/2019).Stability was estimated using additive main effects and multiplicative interaction (AMMI) model.A Combined analysis of variance showed significant effects of genotypes, locations, years and their interactions on seed yield. Mean seed yield ranged between 1.88 and 1.45 t ha-1 for the genotypes FLIP 08-59 C and FLIP 09-187 C, respectively. The genotype FLIP 08-59 C gave the highest average seed yield and out-yielded the two checks, Shiekh Mohamed and Burgeig by 9.5% and 10.1%, respectively. The newly introduced (FLIP 08-59 C) showed non- significant differences in earliness as compared to the other two standard checks resistant to fusarium wilt disease and with good seed weight. The statistical analysis showed that genotype (G), environment (E) and their interaction (GEI) had highly significant (P ≤ 0.001) effect on seed yield. It also revealed that environmental differences accounted for 90.2% of the total variation. The results of data analysis displayed that the percentages of seed yield by environment, genotype and genotype environment interaction were 90.2%, 3.6% and 6.3%, respectively. The IPCA 1 and IPCA 2 axes (Principal Components) were highly significant P ≤ 0.001). The model for seed chickpea yield stability (the Additive Main Effect and Multiplicative Interaction (AMMI) model) showed that the G1 (FLIP 08-59 C) was adapted, high yielding and considered as stable genotype for Gezira, River Nile and Northern States of Sudan. In addition, this genotype is early flowering with non-significant differences in earliness as compared to the two checks and with good seed weight. Therefore, the above mentioned genotype FLIP 08-59 C could be recommended as commercially stable and high yielding cultivar and/or incorporated as breeding stocks in any future breeding programs aiming to produce high yielding genotypes of chickpea.
Author Contributions
Copyright© 2023
E. A. Hamza Fatih, et al.
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Introduction
The chickpea or chick pea ( Chickpea ( The major contribution (almost 86.73 %) of the world production of chickpea is from Asia, with only 5 % coming from Africa. It is reported that the world area under chickpea cultivation is 13.2 million hectares, with a total production of 11.6 million tons In Sudan chickpea is traditionally grown as a winter crop in the northern part, however, its production has expanded recently to the central clay plain of central Sudan. The growing season is restricted to a short period of time by the high temperatures prevailing at the beginning and at the end of the season. Chickpea yield in Sudan varies from 0.83 to 2.8 t/ha, depending on weather conditions genotype and cultural practices Environmental factors, such as soil moisture, sowing time, fertility, temperature and day length have great influences during the various stages of plant growth The differences in genotype stability and adaptability to environment can be qualitatively assessed using the biplot graphical representation that scatters the genotypes according to their principal component values Obviously, low yield is a major problem in the area of Chickpea production. It is caused attributed to several complexes and interacting factors. Therefore, the main objectives of this study are (i) To evaluate the performance of some introduced chickpea genotypes for seed yield and yield related traits under difference environmental conditions in Sudan. (ii) To identify early flowering and fusarium wilt disease resistant genotypes. (iii) To determine the effects of G
Materials And Methods
The research was conducted the 2017/18 and 2018/19 in four locations under irrigation system. Three studies were conducted in northern region of Sudan. One study was conducted at Shendi Research Station Farm (latitude 16° 41' - 16° 45' N, longitude 33° 25' -33° 29'E) in the River Nile State. The second was conducted at Hudeiba Research Station Farm (latitude 17° 33' -17° 37'N, longitude 33° 56' - 33° 59'E) with an average annual rainfall of 74 mm/ann., while the third study was carried out at Merowe locality, in Research Station Farm, (Latitude: 18° 27' - 18° 31' N, Longitude: 31° 49' -31° 53' E, Elevation of 258 meters above the sea level). The three Research Station Farms are located in a desert climatic zone, which is characterized by dry and hot climate. In this region, during the winter, temperatures are warm at the day and cool at night, where the temperature can reach to 0°C. The rest of the year, from May to September is very hot, and the temperature could reach to 40 °C, and sometimes could reach up to 50/52°C. Moreover, the wind can raise sandstorms at any time of the year (Sudan Metrology 2022) Three chickpea genotypes were obtained from the advanced materials of the national chickpea breeding program in the Agricultural Research Corporation (ARC)-Sudan. Moreover, two released chickpea cultivars (Shiekh Mohamed and Burgeig) were used in this study as checks ( Across all growing seasons and locations, the land was well prepared using disc plowing, disc- harrowing, leveling and ridging. The experiments were arranged in a Randomized Complete Block Design (RCBD) with four replicates in all locations. Each genotype was planted in a separate plot composed of five ridges; each of 5 m in length, with plant-to-plant and row-to-row distance of 10 and 60 cm, respectively. Sowing was done in the second week of November during the two winter seasons. Frequent irrigation was carried out at 14-16 days intervals to avoid water stress. The experimental plots were treated with a starter dose of nitrogen in the form of urea at the rate of 43kg N/ha before the fourth irrigation. Two hand weeding was done. Seed yield was assessed from a net area of 8.28 m During the two seasons, data pertaining the Days to 50% flowering (were registered as days from sowing to the date of flowering, when about 50% of the plants bear at least one flower). Hundred-seed weight (g.) (calculated by 100 seed samples which were randomly selected from each plot], seeds weight (seed weighed using a sensitive balance. The latter parameter was recorded after harvesting, threshing and winnowing (in g or kg)). The seed yield (was weighed using electronic balance on net plot basis and later converted into t The five chickpea genotypes were screened for resistance to Fusarium wilt disease in an infested plot at Gezira Research Station Farm during the winter season of 2018/2019, the evaluated for Fusarium wilt disease was done at three stages during the crop cycle (seedling, flowering and pod setting stages). Where disease incidence (%) The collected data were statistically analyzed using the GenStat 12th edition To determine the performance, stability and genotypic superiority across environments or at specific environment, additive main effect and multiplicative interaction (AMMI) model was used
No.
Accession no.
Genetic background (pedigree)
Origin
1
FLIP 08-59 C
X02TH3/FLIP 98-28C X FLIP-97-102C
ICARDA
2
FLIP 09-182 C
X06TH53/FLIP03-128C X FLIP01-25C
ICARDA
3
FLIP 09-187 C
X06TH53/FLIP03-128C X FLIP01-25C
ICARDA
4
Shiekh Mohamed (check)
X99TH62/(FLIP932CxFLIP 94-115C)
Released commercial cultivar
5
Burgeig (check)
ICCC32/(K4/Chafa)
Released commercial cultivar
Results
The results of data analysis for yield and yield related traits in different locations are presented in the MER = Merowe, GEZ = Gezira, SH = Shendi and HU = Hudeiba **and *** = Significant at 0.01and 0.001 levels of probability, respectively. Considering Average seed yield of the five chickpea genotypes across the eight environments varied from 1.45 to/ 1.88 ton/ha. This indicates the wide variability for yield potential among the chickpea genotypes. The three locations, Merowe, Gezira and Shendi, gave the highest seed yield of 2.62, 1.96 and 1.16 ton/ha, respectively. On the other hand, the Hudeiba site recorded the lowest seed yield (0.84 ton/ha). Across all sites, the highest mean seed yield was recorded by the genotype FLIP 08-59,C (1.88 ton/ha) followed by the check Shiekh Mohamed (1.70 ton/ha). The genotype FLIP 08-59 C produced the higher seed yield compared to the check Burgeig at Medani and Hudeiba sites. The same genotype gave significantly higher seed yield than the check Shiekh Mohamed at Shendi site. At Merowe site, the genotype FLIP 08 -59 C produced a comparable seed yield to the check Burgeig. At Gezira site, the genotype FLIP 08-59 C produced the highest seed yield and exceeded the two checks, Burgeig and Shiekh Mohamed by 21.1% and 15.8%, respectively. Whereas at Shendi site, the genotype FLIP 08-59 C recorded the highest yield and out-yielded the two checks, Shiekh Mohamed and Burgeig by 35.9% and 11.1%, respectively. At Hudeiba site, also the genotype FLIP 08-59 produced the higher seed yield and exceeded the two checks Burgeig and Shiekh Mohamed by 18.0% and 6.6%, respectively. The genotype FLIP 09-182 C at Gezira site gave better seed yield and out – yield the two checks Shiekh Mohamed and Burgeig by 8.6% and 14.3%, respectively. **and *** Significant at 0.01and 0.001 levels of probability, respectively. The above MER = Merowe, GEZ = Gezira, SH = Shendi and HU = Hudeiba. **and *** = Significant at 0.01and 0.001 levels of probability, respectively. However, across all sites, there were highly significant differences among genotypes in days to 50% flowering with over all mean of days to 50% flowering, ranging between 39 and 48 days in season 2017/18, whereas ranging between 48 and 42 days in 2018/19. The genotype FLIP 08-59 C was the earliest to reach 50% flowering but with non-significant differences as compared to the two checks. MER = Merowe, GEZ = Gezira, SH = Shendi and HU = Hudeiba *and *** = Significant at 0.05and 0.001 levels of probability, respectively. n.s=not significant at 0.05 level of probability. Regarding the evaluation of Fusarium wilt disease (Fusarium oxysporumf.sp.ciceris), the results summarized in * = Significant at 0.05 level of probability and ns = not significant at 0.05 level of probability. The actual data between parenthesis. R = Resistance and MR = Moderately resistance. The study showed significant differences among the genotypes on fusarium wilt incidence at flowering and podding stages which ranged from 11.3 to 22.2% and 24.4to 39.8%, From the above results, the genotype FLIP 08–59 C was found to the most resistant to Fusarium wilt disease among all the tested genotypes. Regarding the Yield Stability, analysis of variance the study showed high significant differences among the tested genotypes (G) for seed yield, environments (E) and their interactions (GEI) Probability of greater chi-square for testing homogeneity error variances= 0.0336 Error variances are heterogeneous at 0.05 probability. *** = Significant at 0.001 level of probability. n.s: not significant at 0.05 level of probability. The highly significant differences observed among environments indicated that these genotypes were evaluated under diverse seasons and locations. The mean square of G x E interaction also showed high significant differences for most of the traits indicating the effect of the environment and genotype interaction. The mean square of G x E (linear) interaction was not significant. Stability models were followed to carry out the seed yield stability performance for the three tested chickpea genotypes against the two checks. These were the Additive Main Effect and Multiplicative I nteraction GGE biplot model *and *** = Significant at 0.05and 0.001 levels of probability, respectively. n.s= not significant at 0.05 level of probability. For the GGE biplot analysis, the GGE refers to the genotype main effect (G) and the genotype x environment interaction (GE) which are the most important sources of variation for a genotype evaluation in multi environmental trials Rank (RSY)*=rank in seed yield, IPCA 1 and IPCA 2=interaction principal component axis 1 and 2, ASV =AMMI stability value, RASV = rank of AMMI stability value, GSI = genotype selection index. Genotype Selection Index (GSI) measure is essential for quantifying and ranking genotypes according to their yield stability. The one with high seed yield and least (GSI) is considered as the most stable Table 10.presents the best four genotypes in each environment for seed yield. These are the G1, (FLIP 08-59C), and G4 (Shiekh Mohamed), respectively For GGE biplot analysis model, the GGE analysis showed 58.81% and 33.16 % of total variation in the data matrix of GGE respectively, and thus they are accounted for 91.97 % of GGE together ( According to GGE analysis, the genotypes with PC1 scores is close to zero expressed general adaptation, whereas the larger scores are for more specific adaptation to particular environments 29.
FLIP 08-59 C
1.93
2.34
1.41
0.70
1.60
3.39
2.20
1.65
1.40
2.16
FLIP 09-182 C
2.04
2.31
0.71
0.44
1.38
2.56
1.87
1.34
0.93
1.67
FLIP 09-187 C
2.14
1.96
0.82
0.48
1.35
2.79
1.59
1.03
0.78
1.55
Shiekh Mohamed
2.45
2.02
0.89
0.71
1.52
3.41
1.79
1.06
1.25
1.88
Burgeig
2.09
1.67
1.51
0.40
1.41
3.41
1.90
1.21
1.32
1.96
Mean
2.13
2.06
1.07
0.55
1.45
3.11
1.87
1.26
1.14
1.84
S.E ± Years (Y)
0.036***
S.E ± Locations (L)
0.051***
S.E ± Genotypes (G)
0.042**
S.E ± (YxLxG) Interaction
0.071***
C.V (%)
14.4
Year (Season)
1
6.20
6.20***
Location
3
77.45
25.82***
Year x Location
3
7.69
2.56***
Residual
24
2.45
0.10
Genotypes
4
3.60
0.90***
Year x Genotypes
4
0.82
0.21**
Location x Genotype
12
3.13
0.26***
Year x Location x Genotypes
12
2.38
0.20***
Residual
96
5.43
0.06
Total
159
109.15
FLIP 08-59 C
47
42
30
48
42
45
39
50
47
45
FLIP 09-182 C
57
48
31
55
48
52
44
52
46
48
FLIP 09-187 C
48
41
31
48
42
43
44
52
48
47
Shiekh Mohamed
40
41
31
46
39
36
37
50
45
42
Burgeig
46
46
31
47
42
40
40
51
46
44
Mean
48
43
31
49
43
43
41
51
46
45
S.E ± Year (Y)
0.27***
S.E ± Locations (L)
0.382***
S.E ± Genotypes (G)
0.408***
S.E ± (YxLxG) Interaction
1.165**
C.V (%)
5.2
FLIP 08-59 C
22
18
18
19
19
22
22
27
22
23
FLIP 09-182 C
34
31
31
28
31
34
39
30
34
34
FLIP 09-187 C
29
29
27
28
28
30
31
28
27
29
Shiekh Mohamed
25
25
21
24
24
23
24
23
22
23
Burgeig
18
19
19
18
19
21
22
25
20
22
Mean
26
24
23
24
24
26
28
27
25
26
S.E ± Year (Y)
0.3***
S.E ± Locations (L)
0.5*
S.E ± Genotypes (G)
0.5***
S.E ± (YxL) Interaction)
0.7
C.V (%)
12.1
FLIP 08-59 C
(8.8) 17.24a
(5.5) 11.3b
(15.25) 24.4b
R
FLIP 09-182 C
(13.8) 21.34a
(18.0) 22.2a
(41.75) 39.8b
MR
FLIP 09-187 C
(19.2) 25.4a
(10.0) 18.1ab
(35.5) 36.3ab
MR
Shiekh Mohamed
(15.5) 23.0a
(12.5) 20.5ab
(35) 36.1ab
MR
Burgeig
(20.5) 26.5a
(9.0) 17.1ab
(34.25) 35.6ab
MR
Mean
22.7
17.8
34.4
S.E ±
3.24n.s
3.52*
4.80*
C.V (%)
28.6
39.5
28.1
Environment (E)
7
91.37
13.05***
Environment (Linear)
1
91.37
91.37***
Deviations
6
0
0
Residual
24
2.462
0.103
Genotypes (G)
4
3.5937
0.8984***
EG interaction
28
6.3420
0.2265***
EG interaction (Linear)
4
0.3541
0.0885
Pooled Deviations
24
5.9879
0.2495***
Pooled error
96
5.4365
0.0566
Total
159
109.20
Total
159
109.15
0.686
Treatments
39
101.26
2.596***
Genotypes
4
3.6
0.900***
3.6
Environments
7
91.34
13.048***
90.20
Block
24
2.45
0.102*
Interactions
28
6.33
0.226***
6.3
IPCA 1
10
3.72
0.372***
58.8
IPCA 2
8
2.1
0.262***
33.2
Residuals
10
0.51
0.051
Error
96
5.43
0.057
FLIP 08-59 C (G1)
1.88
1
0.22802
0.41499
0.58
2
6
FLIP 09-182 C (G2)
1.53
4
-0.46955
0.37169
0.91
4
5
FLIP 09-187 C (G3)
1.45
5
-0.57229
-0.24218
1.04
5
7
Shiekh Mohamed (G4)
1.70
2
0.16414
-0.02052
0.29
1
4
Burgeig(G5)
1.69
3
0.38015
0.01243
0.67
3
8
Medani 2017
2.061
0.5916
G2
G1
G4
G3
Shendi 2018
1.258
0.204
G1
G2
G5
G4
Hudeiba 2017
0.546
0.1735
G4
G1
G2
G5
Merowe 2017
2.129
0.1352
G4
G3
G1
G5
Medani 2018
1.873
0.0764
G1
G5
G2
G4
Hudeiba 2018
1.135
-0.2124
G1
G5
G4
G3
Shendi 2017
1.069
-0.4351
G1
G5
G4
G2
Merowe 2018
3.114
-0.5333
G5
G4
G1
G3
Conclusion
The main effects of genotype and environment as well as the G×E were highly significant and the environment caused the most variations in seed yield. FLIP 08-59 C is found to be an early flowering genotype compared to the two checks. It lso showed good 100 -seed weight and resistant to Fusarium wilt disease, which is considered among the major biotic stresses to the production of chickpea in Sudan. FLIP 08-59 C produced higher seed yield than all other genotypes overall the environments and performed better at most of the places. It exceeded the two standard checks, Shiekh Mohamed and Burgeig by 9.5% and 10.1%, respectively. FLIP 08-59 C was found to be comparatively stable as its performance was hardly affected by the G x E interaction and thus would perform well across a wide range of environments. This genotype also was found to be more adapted across the different tested environments. Therefore, the genotype FLIP 08-59 C is recommended for release as a cultivar to be grown at Gezira, River Nile and Northern states of Sudan. Also may be used in future in the breeding program for the development of high yielding and stable genotypes.