Abstract
The objective of this study was to evaluate the effectiveness of endophagous Encyrtid parasitic wasp
Author Contributions
Copyright© 2019
Ahmed Mohamed Nawal, et al.
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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Introduction
The species, of Cotton Mealy bug Several researchers' recorded Cotton Mealy bug in Sudan for the first time, after the outbreak in different States of the country during the season 2012/ 2013 The chemical control of Mealy bugs is not only expensive, but it also disturbs the habitats of natural enemies and has negative impacts on different ecosystems and human health Family Encyrtidae (Hymenoptera, Chalcidoidea) is an important Entomophagous group of insects that are parasitoids of a wide range of insects and other Arthropods. Several species of the family have been successfully used as biological control agents in some parts of the world In the United States of America, the parasitoid, Aenasius arizonensis (Girault), Family Encyrtidae (Hymenoptera, Chalcidoidea) was described as Chalcaspis arizonensis One of the researchers In Sudan, the first record of the parasitoid, A. arizonensis was made by researchers Many studies revealed that ants are commonly found in association with Mealy bugs infestation, feeding on honey dew secretions and have long been known to aggravate Mealy bug populations and other honey dew producing insect species by disturbing the natural biological control on these species The present study was carried out to evaluate some of the biological characteristics of this parasitoid, to be applied as a bio-control agent for cotton Mealy bug,
Materials And Methods
A field survey was made to determine the seasonality of Mealy bug, P. Solenopsis and its parasitoid, Aenasius arizonensis. Records were made during the season 2015 - 2016, at two weeks intervals, in three Agricultural Schemes namely; Soba, Gommueia and Selait, which were infested with Mealy bug, P. Solenopsis. The species were identified based on the morphometric features described by the researchers Another field survey was carried out in April, 2019 to study the Infestation of P. solenopsis and parasitism by A. arizonensis at Soba Agricultural Scheme. Where the infestation of P. solenopsis and its parasitized mummies were observed on three different families of weeds, which are locally known as Raba (Trianthema brasilicum), Lissan Al-tair (Amaranthus virids), Hambouk (Abutilon spp). To determine the parasitisation percentage, 50 apical shoots of Hambouk, each of 30 cm in length were collected randomly from five sites. For the laboratory studies, the Mealy bug mummies were collected in plastic jars from ornamental plants, Lantana (Lantana camara) and hibiscus (Hibiscus rosa - sinensis) from Horticulture orchard at the Faculty of Agriculture, Khartoum University, Shambat. They were reared in four transparent plastic jars under laboratory conditions of 28oC temperature and 45% relative humidity so that to allow the emergence of the parasitoid. Breeding colonies of A. arizonensis were established from individuals emerged from the dark mummies. One day old males and females of the emerged parasitoid were allowed to mate for 24 hours. Then, they were transferred by an aspirator to a culture of 30 adult females of the Mealy bugs, reared on Hibiscus After 24 hours, the pair of the parasitoid was taken out from the plastic jar. The exposed Mealy bugs were checked daily until adult parasitoids emerged from the mummified Mealy bugs. Then the following parameters were recorded: mean developmental periods from oviposition to mummy formation, mummy formation to adult emergence, total life span of males and females, sex ratio and mean daily parasitisation. To get more accurate information, the experiment was repeated three (3) times as prescribed by some researchers Data regarding the biology and parasitization of Aenasius arizonensis in the field and the laboratory were statistically analyzed by using Software excel version 2007 and Statistix 8 (Analytical Soft Ware, 2003). Then they were subjected to analysis of variance (ANOVA) under completely randomized design. Means were
compared following the least significant difference test (LSD test) at probability level of 5% to get the mean values of the observations, S.E., parasitism and the Sex ratio from the recorded data.
Results
From It is observed that, the adults emerge from mummies by cutting a circular hole after a pupal period. The emergence holes were found on the posterior– dorsal part of the dorsum of the mummified P. Solenopsis ( Considering the As far as the mean longevity period (from egg to death) for females concern; was 23.37 ±1.89 days and 23± 2.33 days for males, with a range of 13-29 and 13-25 days, for females and males, respectively, which corresponds to the findings of some researchers (2012). The number of parastized Mealy bugs per day ranged from 20- 24, with a mean of 22.5± 0.96 ( During the field surveys, high infestation of Regarding During this study, activities of ants as predators of mummified mealy bugs were observed and also observed carrying about young mealy bug crawlers, aiding in their dispersal (
No.
Month
Host plant species
1
October
Okra
2
November
Okra
3
December
Okra - Lantana ((Figure 1)
4
January
Okra- Hambouk
5
February
China rose (Figure 2) – Hambouk
6
March
Hambouk
7
April
Hambouk
8
May
Ramtouk- Hambouk
9
June
Datura- Hambouk- china rose
10
July
China rose- hambouk
11
August
-
12
September
-
Oviposition to mummy formation
3 ± 1.16
2- 4
Adult male emergence after oviposition
13.29 ± 1.12
10- 16
Adult female emergence after oviposition
13.09 ± 1.02
10-17
Emergence to death for male
9.71 ± 1.21
5- 11
Emergence to death for female
10.28 ± 0.87
9- 14
Pre- oviposition period
>1
>1
Male longevity
23 ± 2.33
13- 25
Female longevity
23.37 ± 1.89
13- 29
Mean of Parasitization (no. of parasitized host / female/ day).
22.5 ± 0.96
20- 24
Sex ratio of progeny (male: female)
1: 1.6
Stage
Mean no. of mummies per 30cm shoot
% parasitism
Total % parasitism
Female
17.908 A
25.02
31.26
Third Nymphal instar
4.24 B
6.24
CV
54.14
SE
0.8479
LSD
1.986
Conclusion
Wasp,