A total of 256 samples were collected from eight different towns of Faisalabad namely: D-Ground, Motorway city, Madina town, Batala colony, Millat town, Iqbal town, Gulberg colony and Ghulam Muhammadabad. The samples were collected by using simple random sampling technique. Samples were divided into four categories as: Egg tray, Egg shell, Egg yolk and albumin. Total 32 samples (8 per category) were collected from each selected town of Faisalabad (Figure 1).

The samples were then carried to laboratory for the Institute of Microbiology, University of Agriculture Faisalabad, under sterile conditions and were stored at 4^oC for further analysis.

Bacteria from nutrient agar (general purpose media) were streaked on MacConkey’s agar, Eosin methylene blue agar, Staphylococcus 110 agar and Salmonella-Shigella agar as shown in Figure 2. Then, biochemical tests were applied for further confirmation of identification. The results are displayed in Table 1. Whereas, glucose, lactose and sucrose fermentation abilities of these bacteria were shown in Figure 3. The result of triple sugar iron fermentation to differentiate between fermenting and non-fermenting bacteria with the ability of acid production from fermentation is displayed in Table 2. Resistance and sensitivity profile of various bacterial isolates against different drugs has been shown in Table 3. Zone of inhibition of various bacterial isolates using different antibiotic disks have been shown in Figure 4.

Poultry is known to be the oldest on earth. It is known for over 150 million years. Poultry species include ducks, geese, turkeys, pheasants, pigeons, peafowl, guinea fowl and chickens. Poultry is important in providing food and fiber to humans in the form of eggs and meat. Among all poultry species chicken is the most commonly used specie for human food consumption. The commercial chicken is the largest industry that provides with eggs and meat 15. Globally, the production of poultry products like eggs and meat is rapidly increasing. This reflects that the consumption of these products has also been increased. According to different researches an overall consumption of poultry products from 1995 to 2005 has increased globally with the ratio of 53% for chicken meat, 13% for turkey meat, 67% for duck meat, 53% for goose meat, 39% for chicken eggs and 27% for other eggs. Therefore, it is important to know that what types of contaminants may be present in these products and what risks they can cause to human health 16. Microbes depreciate the quality of eggs, impairs growth and decreases the performance of birds 8.

Antibiotic resistance is a natural phenomenon. Data regarding the prevalence of antibiotic resistance and the types of ESBLs produced by E. coli are available for certain regions of China and from many other countries 1718. In a study conducted in China, ninety-seven percent of the E. coli isolates were resistant to at least three different classes of antibiotics (MDR), whereas 18% were resistant to all antimicrobials tested 1819. Isolation, identification and characterization of commonly occurring egg borne pathogens including E. coli, Salmonella entritidis, Bacillus subtilis, Staphylococcus aureus was carried out by different cultural, morphological and biochemical tests. For culturing these microbes different culture media were used that include: Nutrient agar, Salmonella-shigella agar, MacConkey agar, Eosin-methylene blue agar. Antibiotic resistance pattern of recovered bacterial pathogens were determined by disk diffusion method (Kirby-Bauer). Zone of inhibition was measured through vernier calliper. Data was analyzed by Analysis of variance. The results revealed a high prevalence of Salmonella entitidis with 40.83% following Staphylococcus aureus with 22.08%, Escherichia coli with 17.92% and Bacillus subtilis in a lower ratio with 2.5%. This showed that Bacillus subtilis is present in a very low amount in chicken egg. The antibiotic sensitivity test showed different resistance patterns of the isolated microbes. Bacillus subtilis was highly resistant to penicillin and cefotaxime while it sensitivity for gentamicin, tetracycline and levofloxacin. E. coli was sensitive to gentamicine and levofloxacine while it showed resistance to other three antibiotics that were tetracycline, cefotaxime and penicillin. Staphylococcus aureus was sensitive to different drugs naming penicillin, gentamicin, tetracycline and levofloxacine. The only drug to which Staphylococcus aureus showed resistance was cefotaxime. Salmonella was resistant to tetracycline and gentamicin. Thus, proper attention should be paid to cope up with possible future egg-borne illnesses as in present study chicken eggs have been found contaminated with different pathogenic or non-pathogenic bacterias.